Background Eye drops made from platelet-rich plasma are used in the treatment of ocular surface dysfunctions after LASIK refractive surgery, severe dry eye and corneal ulcers.
Purpose To describe the process of enriching the plasma, and the dosage used.
Materials and Methods This master formula was developed from the information obtained from a literature search in PubMed and Embase.
Results We extracted blood in 10 ml tubes containing 3.2% sodium citrate. The amount required depends on the length of treatment. From each 10 ml of blood processed, we obtained 3–4 ml of platelet-rich plasma, sufficient for one week of treatment. We received 10 tubes of blood from each patient. These were centrifuged at a speed of 1400 rpm for 10 minutes to obtain maximum concentration. The tubes were kept in an upright position, to avoid mixing the contents and waste. The eye drops were prepared in sterile conditions in a laminar flow cabinet. Using a sterile Pasteur pipette, we removed the top layer of the centrifuged blood which is the platelet-rich plasma. The plasma was collected in syringes and was then stored in sterile light-resistant containers, each containing 2–3 ml of platelet-rich plasma. It remained stable for a week in a fridge, or 3 months in a freezer. Patients were treated with a dose of 5–10 drops per day. The duration varied according to the diagnosis (between 1 week and 1 month of treatment).
Conclusions Platelet-rich plasma eye drops made in the Pharmacy Service after consulting previously published research and according to Royal Decree 1751/2001 are a new and alternative treatment for corneal ulcers, dry eye and post-LASIK dysfunction of the ocular surface.
No conflict of interest.
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