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CP-143 Involvement of microbial flora in aetiology of surgical site infections
  1. CI Staicus1,
  2. D Calina2,
  3. L Rosu3,
  4. AF Rosu3,
  5. O Zlatian3
  1. 1University of Medicine and Pharmacy, Faculty of Medicine, Craiova, Romania
  2. 2Faculty of Pharmacy, Clinical Pharmacy Department, Craiova, Romania
  3. 3Faculty of Medicine, Microbiology Department, Craiova, Romania

Abstract

Background The most common complications of surgical wounds are abscesses and necrosis.

Purpose To establish the aetiology of surgical wound infections in post-surgery patients from the hospital and to assess the resistance of the germs isolated to antimicrobials. Variations in antibiotic resistance profile in relation to the type of suppuration and surgical wards were compared in order to establish the most effective therapeutic protocols.

Material and methods From 165 hospitalised and ambulatory patients we collected: pus from abscesses/phlegmons, ear, nose and throat suppurations, discharge from superficial and deep surgical wounds. We performed classical bacteriological diagnosis and Kirby Bauer testing.

Results Staphylococcus aureus was isolated in 41.15% of cases, coagulase-negative staphylococci in 4.31% and in the remainder Gram-negative bacilli: Klebsiella spp. (13.88%), Escherichia coli (13.40%), Pseudomonas (8.13%), Proteus (6.22%), non-fermentative Gram-negative bacilli (4.78%), Enterobacter (1.91%) and Citrobacter (0.48%).

Staphylococcus aureus was resistant to ceftriaxone (100%), penicillin (91.36%), amoxicillin (83.33%), amikacin (80.00%) and ampicillin (67.92%). E. coli strains were resistant to chloramphenicol (100.00%), amoxicillin clavulanate (87.50%) and ampicillin (64.50%). Klebsiella was resistant to ampicillin (100%), amoxicillin clavulanate (87.50%) and amikacin (50.00%). Pseudomonas was found to be highly resistant to cefpirome (87.50%), meropenem (66.67%) and tigecycline (66.67%).

36.84% of all strains were resistant to more than five antibiotics. The average resistance index of strains isolated from the superficial suppurations was higher than those isolated from the deep suppurations (Student’s t = −3.025, p = 0.0014). The resistance index also indicated that the strains isolated from hospitalised patients were more resistant than those from ambulatory patients (Student’s t = −3.4237, p = 0.0008).

Conclusion This study shows the prevalence of multidrug resistant strains in our hospital and their involvement in surgical wound infections. Continuous microbiological surveillance of germs isolated from surgical suppurations and their resistance to antimicrobials is essential for defining antibacterial policies on surgical wards.

References and/or Acknowledgements No conflict of interest.

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