Background Platelet-rich plasma (PRP) has been shown to clinically accelerate the healing of both soft and hard tissues, although its analgesic and anti-inflamatory (AA) activity yields in its concentration on blood-cell counts and certain grown factors. In 2013 PRP changed it condition and aquired the classification of ‘medicine’ by the drug authorities. Preparation in an open manner is allowed under certain conditions, although techniques are not standarised for its composition.

Purpose We sought to describe and analyse our PRP prepared in our facilities.

Material and methods Following GMP practice guidelines, PRP was manufactered under an open technique. 100 g for 10 min conditions were applied. For each patient, 70 ml of peripheral blood were extracted and 14 ml of PRP was obtained. Cell counts and the contents of vascular endothelial growth factor (VEGF), platelet-derived growth factor AB (PDGF-AB), transforming growth factor beta 1 (TGF-b1), interlekin beta 1 (IL-B1) and insulin growth factor (IGF) concentration of growth factors in PRP were analysed.

Results Seventy-four patients were included. In table 1, peripheral blood sample and PRP composition are shown. Concentration and percentage recovery were 2.28 (2.15–2.36) and 45.6 (43.15–47.14) for platelets; 0.45 (0.39–0.6) and 9.17 (7.83–11.93) for white cells; and 0.01 (0.01–0.01) and 0.22 (0.18–0.29) for red cells, respectively.

Conclusion We obtained high concentration and percentage recovery rates of platelets and poorer rates for the rest of the blood cells (optimal effect) and a significant amount of the pre-selected grown factors proved to be involved in the AA effect of PRP.

References and/or acknowledgements None.

No conflict of interest.