Background In severe infections such as in intensive care units (ICUs), the recommended dose of vancomycin may be 60 mg/kg/day. Studies demonstrated that continuous infusion of vancomycin allowed rapid target concentration. In ICUs, a minimum volume is used to avoid fluid overload for patients requiring fluid restriction, leading to high concentrations of vancomycin.
Purpose The first objective of this work was to study the impact of an electric syringe pump on physical stability. The second objective was to study the stability of vancomycin solutions at 62.5 mg/mL and 83.3 mg/mL, diluted in two solvents: 0.9% sodium chloride or 5% glucose, in polypropylene syringes after the preparation and after a 6 hour, 24 hour and 48 hour storage at room temperature.
Material and methods Chemical stability was analysed by high-performance liquid chromatography coupled to a photodiode array detector at each time of analysis. The method was validated according to the International Conference on Harmonisation Q2 (R1). Physical stability was evaluated by visual and subvisual inspection (turbidimetry by UV spectrophotometry at 350, 410 and 550 nm as recommended by the European Consensus Conference). Three syringes for each condition were prepared. At each time of analysis, three samples were analysed for each syringe. pH and osmolality values were evaluated at each moment of the analysis. Chemical stability was defined as not less than 90% of the initial concentration.
Results The action of an electric syringe pump did not cause visual modification. Vancomycin diluted in 0.9% sodium chloride at 62.5 mg/mL and at 83.3 mg/mL retained more than 90% of the initial concentration after 48 hours and 24 hours respectively. Diluted in 5% glucose and stored at 20°C–25°C, vancomycin solutions at 62.5 mg/mL and 83.3 mg/mL retained more than 90% of the initial concentration after 48 hours. Some precipitates were visible after 48 hour storage for the vancomycin syringe at 83.3 mg/mL in 0.9% sodium chloride. In other conditions, no visual modification was observed.
Conclusion Vancomycin hydrochloride diluted in 5% glucose at 62.5 mg/mL and 83.3 mg/mL were physically and chemically stable over a period of 48 hours at room temperature. For high concentrations of vancomycin, 5% glucose as solvent is recommended.
References and/or acknowledgements No conflict of interest.
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