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6ER-025 Antimicrobial activity of subcritical CO2 extract obtained from underground Ferula asafoetida L
  1. U Datkhayev,
  2. K Zhakipbekov,
  3. N Rakhymbayev,
  4. S Mombekov,
  5. M Ashirov,
  6. A Daulbayeva,
  7. N Zhumabayev
  1. Asfendiyarov Kazakh National Medical University, School of Pharmacy, Almaty, Kazakhstan


Background and Importance Literature review showed that various extracts of Ferula asafoetida L. have wound healing, anti-inflammatory, antinociceptive, antimicrobial, antitumour, antidiabetic effects. However, there is a lack of studies regarding CO2 extracts of Ferula asafoetida L. This raises the need for phytochemical and antimicrobial study of this extract.

Aim and Objectives The possibility of creating to consider an antimicrobial preparation based on CO2 extract of Ferula asafoetida L. used in pharmacy practice.

Material and Methods Determination of the constituents of the CO2 extract of Ferula asafoetida L. was done by GC-MS and identified by comparing the obtained spectra with the existing NIST library.

Results GC-MS analysis of the CO2 extract of Ferula asafoetida L. showed that some components of sulfur compounds (34.69%) were in rather high concentrations. In the course of the studies, the minimum inhibitory concentrations (MIC) of the CO2 extract of Ferula asafoetida L. were determined by the method of serial dilutions in liquid nutrient medium: Staphylococcus aureus 7.81 µg/ml, Bacillus subtilis 31.25 µg/ml, Escherichia coli, Klebsiella pneumoniae, Salmonella enterica 15.63 µg/ml, Candida albicans, Aspergillus niger 62.5 µg/ml . In the second method, the CO2 extract of Ferula asafoetida L. is more active than the comparison drug amoxicillin against Staphylococcus aureus and spore bacterium Bacillus subtilis by 1.2-fold, Escherichia coli by 1.5-fold and Salmonella enterica by 1.4-fold. And also this extract showed fungicidal activity against Candida albicans 1.5 times more than fluconazole.

Conclusion and Relevance The wide range of antimicrobial properties of the CO2 extract of Ferula asafoetida L. is associated with the presence of sulfur compounds in its chemical composition. As a result of comparing the antimicrobial activity of this extract with literature data, we found that the antimicrobial activity of CO2-extract of Ferula asafoetida L. is higher than that of polar extracts of this plant, and that of essential oils it is higher against Escherichia coli and Bacillus subtilis. In view of the above, the CO2-extract of Ferula asafoetida L. can be used in pharmaceutical practice as a medicinal herbal remedy with antimicrobial action.

References and/or Acknowledgements The authors would like to thank the staff of microbiological laboratories of the Scientific Centre for Anti-Infectious Drugs for their support in this work.

Conflict of Interest No conflict of interest.

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