PT - JOURNAL ARTICLE AU - L. Hoyo Gil AU - S. Sánchez Suarez AU - A. Martín Bravo AU - L. Sastre Gallego AU - N. López Ferrando TI - Pharmacological vitrectomy with urokinase: description of the method and review of a case series. AID - 10.1136/ejhpharm-2012-000074.390 DP - 2012 Apr 01 TA - European Journal of Hospital Pharmacy: Science and Practice PG - 234--234 VI - 19 IP - 2 4099 - http://ejhp.bmj.com/content/19/2/234.1.short 4100 - http://ejhp.bmj.com/content/19/2/234.1.full SO - Eur J Hosp Pharm2012 Apr 01; 19 AB - Background Pharmacological vitrectomy (PV) with autologous plasmin is used to detach the vitreous, with or without subsequent surgical vitrectomy, in several pathologies such as proliferative diabetic retinopathy, proliferative vitreoretinopathy, macular hole, posterior hyaloid contracture syndrome and vitreomacular traction syndrome. Autologous plasmin is obtained by an expensive and complicated method. An alternative method is the use of urokinase as an enzymatic activator of the plasmin; it is cheap and simple to make. Purpose To describe the technique and our experience with it during the first year of use in our hospital. Materials and methods The steps are as follows: 1- Take 7 ml of blood from the patient, place in the centrifuge tube and centrifuge at 4,000 rpm for 15 min. Simultaneously, a phial of urokinase is heated for 15 min at 37°C. 2- Mix 1.8 ml of plasmin with 0.2 ml of urokinase, shaking the mixture vigorously for another 2-3 min, keeping the mixture in incubation at 37°C until use. 3- Sterilise the solution by filtration through a 0.22 mm filter, immediately preceding the injection of 0.2 ml into the eye. Results Over this year using this technique in our hospital 17 patients have been treated with it, in 3 of them the procedure had to be repeated. An improvement in visual acuity was observed in 62.5% of these patients one month after the intervention but was not associated with an improvement in retinal anatomy. Conclusions PV is a cheaper, faster and easier technique than the operation used prior to PV. PV is an interesting technique to perform in hospitals that do not have retinal surgery or in patients with comorbidities that contraindicate vitrectomy under anaesthesia.