Comparison of the influence of acetonitrile (ACN) concentration on chromatographic parameters
| Retention time | Peak symmetry | |||
|---|---|---|---|---|
| ACN concentration in the mobile phase | Bupivacaine | Fentanyl | Bupivacaine | Fentanyl |
| 20% | 13.32 | 26.61 | 4.76 | 2.17 |
| 25% | 11.83 | 20.86 | 4.21 | 1.96 |
| 30% | 10.42 | 16.26 | 3.51 | 1.73 |
| 35% | 10.36 | 15.98 | 3.34 | 1.66 |