Table 4

Results of the high performance liquid chromatography determinations of drug concentrations in the remifentanil–human insulin–propofol combination. Considering the extensive handling required for assaying these three drugs, they were measured only at T0 and T24 in test tubes and not with dynamic simulation

		% of expected concentration in test tube
Active drug	Simulated flow rate	0 h	24 h	Relative difference (%)
Remifentanil	1 ml/h	107.1	104.0	−2.97
Propofol	1 ml/h	97.0	98.7	1.74
Insulin	1 ml/h	105.9	105.9	0.00
Maintenance infusion	1 ml/h

Remifentanil	1 ml/h	95.5	94.5	−1.14
Propofol	1 ml/h	103.4	100.5	−2.84
Insulin	4 ml/h	102.4	105.0	2.47
Maintenance infusion	1 ml/h

Remifentanil	1 ml/h	105.5	103.5	−1.85
Propofol	4 ml/h	98.5	89.2	−9.91
Insulin	1 ml/h	96.8	99.1	2.26
Maintenance infusion	1 ml/h

Remifentanil	1 ml/h	96.3	96.8	0.49
Propofol	4 ml/h	99.5	94.2	−5.45
Insulin	4 ml/h	98.9	97.2	−1.69
Maintenance infusion	1 ml/h

Remifentanil	4 ml/h	100.7	97.5	−3.18
Propofol	1 ml/h	108.5	100.2	−7.95
Insulin	1 ml/h	98.1	98.9	0.83
Maintenance infusion	1 ml/h

Remifentanil	4 ml/h	104.3	101.3	−2.85
Propofol	1 ml/h	109.4	104.9	−4.20
Insulin	4 ml/h	100.0	95.1	−5.01
Maintenance infusion	1 ml/h

Remifentanil	4 ml/h	103.7	105.4	1.61
Propofol	4 ml/h	102.6	93.4	−9.43
Insulin	1 ml/h	91.6	93.0	1.58
Maintenance infusion	1 ml/h