Validation of HPLC method
| Compound | Concentration tested (mg/L) | Recovery (%) | Precision RSD (%) | |
|---|---|---|---|---|
| Intra-day | Inter-day (5 days) | |||
| Butorphanol tartrate | 5.0 | 100.2 | 1.3 | 2.4 |
| 15.0 | 98.5 | 0.7 | 1.1 | |
| 50.0 | 99.7 | 1.2 | 1.6 | |
| Ropivacaine hydrochloride | 200.0 | 100.3 | 0.9 | 1.2 |
| 800.0 | 98.7 | 0.5 | 2.1 | |
| 1200.0 | 99.6 | 1.0 | 1.5 | |
HPLC, high-pressure liquid chromatography; RSD, relative SD.