Chromatographic conditions of methods
| Analyte | Mobile phase | λ (nm) | Calibration range (mg/mL) | Flow (mL/min) | Temperature (°C) | Injection volume (mL) |
|---|---|---|---|---|---|---|
| Voriconazole | 60% Buffer (KH2PO40.05 M pH=3.5)/40% acetonitrile | 254 | 1–7 | 1 | 25 | 40 |
| Isavuconazole | 50% Buffer (KH2PO40.05 M pH=3.5)/50% acetonitrile | 260 | 0.5–10 | 1 | 25 | 50 |
| Posaconazole | 50% Buffer (KH2PO40.05 M pH=3.5)/50% acetonitrile | 260 | 0.3–1.5 | 1 | 25 | 50 |