Chromatographics conditions and calibration methods
| Analyte | Mobile Phase | λ nm | Calib````````on range | Flow (mL/min) | Temperature (°C) | Injection volume (µL) |
|---|---|---|---|---|---|---|
| Linezolid | 75% BUFFER (KH2PO4 20 nM Ph=3.5)/25% acetonitrile | 260 | - 6 calibration levels: range 1–14 µg/mL -n=5 replicates/calibration levels) - Blank (no analyte, no IS) - Zero calibrator (blank + IS) | 1.5 | 25°C | 50 |