Aggregation and denaturation of antibodies: a capillary electrophoresis, dynamic light scattering, and aqueous two-phase partitioning study

J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 25;807(1):17-24. doi: 10.1016/j.jchromb.2004.01.029.

Abstract

Protein denaturation and aggregation are well-known problems in the pharmaceutical industry. As the protein aggregates, it loses its biological activity and creates problems in its administration to patients. In this paper, we explore the use of aqueous two-phase systems, capillary zone electrophoresis, and dynamic light scattering for the monitoring of protein denaturation and aggregation. Our studies focus on human IgG and HSA. Capillary zone electrophoresis was used to monitor changes in the charge to size ratio of the proteins upon denaturation and dynamic light scattering was used to detect the presence of any aggregates and to monitor the size of the proteins. The information obtained from aqueous two-phase partitioning is similar to that obtained from capillary zone electrophoresis. The simplicity of aqueous two-phase system and its low cost (compared to the other analytical techniques) suggest that it can be routinely used for the quality control of some pharmaceutical preparations.

MeSH terms

  • Antibodies / chemistry
  • Antibodies / isolation & purification*
  • Electrophoresis, Capillary / methods*
  • Light
  • Protein Denaturation
  • Scattering, Radiation

Substances

  • Antibodies