Abstract
The immuno-pharmacological profile of a novel immunosuppressive agent, FK-506 produced by a streptomycete, is presented here. We proceeded to test the effect of the agent on various in vitro immune systems. It showed that mixed lymphocyte reaction, cytotoxic T cell generation, the production of T cell-derived soluble mediators such as interleukin 2 (IL-2), interleukin 3 and gamma-interferon and the expression of the IL-2 receptor were suppressed by this agent. The IC50 values of FK-506 and ciclosporin (CS) in all tests were approximately 0.1 nM and 10 nM, respectively. Therefore, the novel agent, FK-506 suppressed in vitro immune systems at about hundred times lower concentration than CS.
MeSH terms
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Animals
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Cyclosporins / pharmacology
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Cytotoxicity, Immunologic / drug effects
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Female
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Humans
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Immunosuppressive Agents / isolation & purification
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Immunosuppressive Agents / pharmacology*
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In Vitro Techniques
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Interferon-gamma / biosynthesis
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Lymphocyte Culture Test, Mixed
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Lymphocytes / drug effects
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Prednisolone / pharmacology
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Pyridines / isolation & purification
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Pyridines / pharmacology
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Receptors, Immunologic / drug effects
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Receptors, Interleukin-2
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Streptomyces / analysis*
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T-Lymphocytes, Cytotoxic / drug effects
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Tacrolimus
Substances
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Cyclosporins
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Immunosuppressive Agents
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Pyridines
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Receptors, Immunologic
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Receptors, Interleukin-2
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Interferon-gamma
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Prednisolone
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Tacrolimus